STAT1 antibody (pTyr701) (Alexa Fluor 647)

Catalog No. ABIN1177190

There are 6+ publications for this product available. The Mouse Monoclonal anti-STAT1 antibody is suitable to detect STAT1 in samples from Human and Mouse. It has been validated for ICS.

Quick Overview for STAT1 antibody (pTyr701) (Alexa Fluor 647) (ABIN1177190)

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT1 antibody is conjugated to Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: 4a

Product Details anti-STAT1 Antibody (Alexa Fluor 647)

Binding Specificity: pTyr701

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Stat1 Peptide

Isotype: IgG2a

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-STAT1 antibody (Alexa Fluor 647) (ABIN1177190)

Tanaka, Saito, Kunisawa, Kurashima, Wake, Suzuki, Shultz, Kiyono, Ishikawa: "Development of mature and functional human myeloid subsets in hematopoietic stem cell-engrafted NOD/SCID/IL2rγKO mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 12, pp. 6145-55, (2012) (PubMed).

Suni, Maino: "Flow cytometric analysis of cell signaling proteins." in: Methods in molecular biology (Clifton, N.J.), Vol. 717, pp. 155-69, (2011) (PubMed).

Perez, Mitchell, Campos, Gao, Li, Nolan: "Multiparameter analysis of intracellular phosphoepitopes in immunophenotyped cell populations by flow cytometry." in: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, (2000) (PubMed).

Darnell: "STATs and gene regulation." in: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, (1997) (PubMed).

Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." in: Cell, Vol. 74, Issue 6, pp. 1135-45, (1993) (PubMed).

Target Details for STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Alternative Name: Stat1

Background: Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84-kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs). The 4a monoclonal antibody recognizes the phosphorylated Y701 in Stat1alpha and Stat1beta.

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events