DDB2
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
1F11
unconjugated
Application Notes
Western blot (1/500-1/1,000 - a 47.8 kDa band corresponding to human DDB2 is detected. Most cell lines expressing DDB2 can be used as a positive control). ELISA (1/2,000-1/10,000). Immunoprecipitation (The antibody immunoprecipitates in vitro translated protein andprotein from cell lysates (using 293T, and others). Coimmunoprecipitation of relatedproteins has not been tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
85.0 mg/mL (by refractometry)
Handling Advice
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage
4 °C/-20 °C
Storage Comment
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Luijsterburg, Goedhart, Moser, Kool, Geverts, Houtsmuller, Mullenders, Vermeulen, van Driel: "Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC." in: Journal of cell science, Vol. 120, Issue Pt 15, pp. 2706-16, (2007) (PubMed).
Target
DDB2
(Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))
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Background
The DDB2 gene encodes the small subunit (p48) of DNA damage-binding protein, which is a heterodimer, composed of a large (p127 DDB1) and a small subunit. The DDB2 subunit appears to be required for DNA binding. This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the large subunit.Synonyms: DDB p48 subunit, DDBb, DNA damage-binding protein 2, Damage-specific DNA-binding protein 2, UV-DDB2, UV-damaged DNA-binding protein 2