Mouse anti-Rat IgE (Chain epsilon) Antibody
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- Target See all IgE products
- IgE
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Binding Specificity
- Chain epsilon
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Purification
- Affinity chromatography on IR2 IgE rat myeloma
- Immunogen
- IR162, IR1016, IR2 and IR410 rat IgE myeloma proteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Clone
- MARE-1
- Isotype
- IgG1
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- Application Notes
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ELISA: 5 μg/mL, This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgE (SA013). Western Blot.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Comment
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This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgE (SA013).
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
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- Target
- IgE
- Abstract
- IgE Products
- Background
- Synonyms: Rat Immunoglobulin E
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