This antibody recognises the integrin alpha2 subunit, which is a 160 kDa glycoprotein that non-covalently associates with the 130 kDa integrin beta1 subunit to form the VLA-2 complex. CD49b is expressed by platelets, long term cultivated T cells, endothelial cells, monocytes and most adherent cell lines. Cross reacts with Cynomolgus monkey (3), Baboon and Rhesus monkey.
Purification
Affinity chromatography on Protein G
Immunogen
Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
ITGA2
Reactivity: Human, Rat
WB, IHC, ELISA, ICC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/500 - 1/1000. Recommended positive control tissue: Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD49b is a 160kD glycoprotein that non-covalently associates with the 130kD integrin beta 1 subunit to form the VLA-2 complex. CD49b is expressed by platelets, long term cultivated T cells, approximately 50 % of monocytes and most adherent cell lines. MCA743 inhibits cell attachment to collagen.Synonyms: CD49 antigen-like family member B, Collagen Receptor, GPIa, ITGA-2, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2 alpha, VLA2