CD56 antibody (Biotin)

Catalog No. ABIN118741

Product Details anti-CD56 Antibody (Biotin)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD56 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: Recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM) (1).

Immunogen: Human retinoblastoma tumor tissue, membrane fraction

Clone: ERIC-1

Isotype: IgG1

Application Details

Application Notes: Studies of neural cell adhesion molecule. May also be used to study isoforms of NCAM expressed on different tumor types. Western blot for analysis of isoforms expressed, did not react with any of the leukemias or lymphomas tested (1). Flow cytometric applications (1 μg to stain 6 x 10e5 cells). Not for use in IHC with formalin-fixed, paraffin embedded sections. The binding of ERIC-1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (-20 °C) for 5 minutes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 100 µg/mL (OD280nm)

Buffer: 0.01 M PBS, pH 7.2, containing 2.0 mM EDTA, 1 % BSA as stabilizer and 0.09 % Sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: CD56 (NCAM1 Products)

Synonyms: CD56 antibody, MSK39 antibody, NCAM antibody, fj56g09 antibody, ncam antibody, ncam1 antibody, wu:fj56g09 antibody, NCAM1 antibody, n-cam antibody, E-NCAM antibody, NCAM-1 antibody, Ncam antibody, Cd56 antibody, N-CAM antibody, N-CAM-1 antibody, NCAM-C antibody, NCAMC antibody, N-CAM-1-B antibody, NCAM-1-B antibody, ncam1-a antibody, ncam1-b antibody, neural cell adhesion molecule 1 antibody, neural cell adhesion molecule 1a antibody, neural cell adhesion molecule 1 S homeolog antibody, neural cell adhesion molecule 1 L homeolog antibody, NCAM1 antibody, ncam1a antibody, ncam1 antibody, LOC100085373 antibody, ncam1.S antibody, Ncam1 antibody, ncam1.L antibody

Background: NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.Synonyms: N-CAM-1, NCAM, NCAM-1, Neural cell adhesion molecule 1

Gene ID: 4684

NCBI Accession: NP_000606

UniProt: P13591