This Antibody is specific for the β subunit of LFA-1. This antibody also recognizes MAC-1 and p150, 95 as well as LFA-1, since they all contain the same β subunit. It inhibits homeotypic aggregation of PHA blasts and blocks the binding of rat lymphocytes to purified rat ICAM-1
Immunoprecipitation. Flow cytometry. Immunohistochemistry on cryostat sections. Functional studies: in vivo/in vitro. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS, pH 7.2, with 0.09 % NaN3 and 0.5 % BSA.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
LFA-1 (lymphocyte function associated molecule-1) is one of the leukocyte integrins. It is a heterodimer consisting of α and β subunits of 160-170 kDa and 95-100 kDa respectively. LFA-1 promotes non-antigen dependent adhesion of T-cells to a variety of lymphoid cells that bear its complementary receptor I-CAM-1 (1). It has a broad distribution and is found on most common lymphocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7