Integrin beta 2 antibody

Catalog No. ABIN120420

The Mouse Monoclonal anti-Integrin beta 2 antibody (Clone WT-3) (ABIN120420) specifically detects Integrin beta 2 in FACS, WB, IP and Func. The antibody is reactive with Rat samples.

Quick Overview for Integrin beta 2 antibody (ABIN120420)

Target: Integrin beta 2 (ITGB2)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)

Clone: WT-3

Product Details anti-Integrin beta 2 Antibody

Specificity: This antibody reacts with the beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits. Clone WT.3 has been shown to inhibit homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1. SM264A is recommended for this purpose.

Purification: Affinity chromatography on Protein G

Immunogen: FTL-43. Lymph node cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: CD18 / ITGB2

Background: CD18 is expressed by all leucocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gene ID: 25021

NCBI Accession: NP_036843

UniProt: Q9JI30

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation