Collagen Type I antibody

Catalog No. ABIN121921

Product Details anti-Collagen Type I Antibody

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognises human Type 1 collagen. It has been cross-adsorbed against collagen types II, III, IV, V and VI. Results from ELISA demonstrate < 10 % cross-reactivity with other collagen types.

Characteristics: Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen

Purification: Affinity chromatography

Immunogen: Human type 1 collagen

Application Details

Application Notes: Immunohistochemistry on frozen sections (1: 10 - 1: 20). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: 100 mM Borate buffered saline, pH 8.0, without preservatives and stabilizers.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store (in aliquots, undiluted) at -20 °C. Avoid repeated freezing and thawing. Should thisproduct contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I (COL1 Products)

Synonyms: collagen type III alpha 1 chain antibody, COL3A1 antibody

Background: Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 1277

UniProt: P02452