GSK3 alpha antibody

Catalog No. ABIN126803

The Mouse Monoclonal anti-GSK3 alpha antibody is suitable to detect GSK3 alpha in samples from Human, Mouse, Rat and Dog. It has been validated for WB.

Quick Overview for GSK3 alpha antibody (ABIN126803)

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This GSK3 alpha antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 10C1

Product Details anti-GSK3 alpha Antibody

Specificity: This antibody specifically recognizes GSK3-alpha phosphorylation independent at 51 kDa. Mab GSK3a-10C1 does not crossreact with GSK3-beta.

Characteristics: Synonyms: Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography

Components: incl. positive Control

Immunogen: Peptide conjugated to hemocyanin

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide/PEG and Sucrose/50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Alternative Name: GSK3 alpha

Background: Glycogen Synthase Kinase 3 (GSK3) exists in two isoforms, alpha and beta (GSK3alpha, GSK3beta). GSK3 is a serine/threonine kinase that becomes phosphorylated by PKB/akt upon activation of the PI3K signal transduction pathway. Phosphorylation of GSK3alpha at serine 21 or GSK3beta at serine 9, respectively, leads to inhibition of kinase activity of GSK3.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha

Molecular Weight: 51 kDa

Gene ID: 2931

UniProt: P49840

Pathways: PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect