GST antibody

Catalog No. ABIN126810

The Mouse Monoclonal anti-GST antibody (Clone 4H3) (ABIN126810) specifically detects GST in WB, IP and EIA. The antibody is reactive with Schistosoma japonicum samples.

Quick Overview for GST antibody (ABIN126810)

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Mouse

Clonality: Monoclonal

Conjugate: This GST antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 4H3

Product Details anti-GST Antibody

Characteristics: Synonyms: Glutathione S-transferase Tag, GST26-Tag

Purification: Size exclusion chromatography

Immunogen: Recombinant GST (pGEX), Schistosoma japonicum

Isotype: IgG2a

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: Glutathione-S-Transferase (GST)

Background: The glutathione-S-transferase (GST) of Schistosoma japonicum is widely used as fusion partner in protein expression systems. The GST can be used for affinity purification of fusion proteins on immobilized glutathion as well as tag sequence if antibodies specific for the expressed protein are not available.Synonyms: GST26-Tag, Glutathione S-transferase Tag

UniProt: P08515