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AKT1 antibody (AA 107-122)
AKT1
Reactivity: Human, Rat, Dog
WB, EIA, IHC (fro)
Host: Mouse
Monoclonal
5G12
unconjugated
Product Details anti-AKT1 Antibody
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Target
See all AKT1 Antibodies
AKT1
(V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
Binding Specificity
All epitopes for AKT1 antibodies
AA 107-122
Reactivity
All reactivities for AKT1 antibodies
Human, Rat, Dog
Host
All hosts for AKT1 antibodies
Mouse
Clonality
All clonalities for AKT1 antibodies
Monoclonal
Conjugate
All conjugates for AKT1 antibodies
This AKT1 antibody is un-conjugated
Application
All applications for AKT1 antibodies
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
This antibody specifically recognizes the alpha isoform of PKB (PKBα/Akt1).
Characteristics
Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase
Purification
Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Components
incl. positive Control
Immunogen
Synthetic peptide conjugated to KLH. Epitope: aa 107-122
Clone
5G12
Isotype
IgG1
Top Product
Discover our top product AKT1 Primary Antibody
Alternatives
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Application Details
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Application Notes
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Recommended positive control: Cell lysate from untreated Neuro2A cells. The antiboby crossreacts in immunoblot applications with an unknown protein of ca.120 kDa expressed in human tumor cell lines. ELISA: 0.05 μg/mL. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Concentration
0.1 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide, PEG and Sucrose/50 % Glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store the antibody at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target Details for AKT1
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Target
AKT1
(V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
Alternative Name
AKT1 / PKB (AKT1 Products )
Synonyms
AKT antibody, CWS6 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, Akt antibody, Rac antibody, PKB/Akt antibody, PKBalpha antibody, AKT/PKB antibody, AKT1 antibody, Akt/PKB antibody, Akt1 antibody, CG4006 antibody, D-Akt antibody, DAKT1 antibody, DAKT1/PKB antibody, DAkt antibody, DAkt1 antibody, DPKB antibody, DRAC-PK antibody, DRAC-PK66 antibody, DRAC-PK85 antibody, Dakt antibody, Dakt1 antibody, Dmel\\CG4006 antibody, Dpkb antibody, PKB/AKT antibody, PKB/dAKT antibody, RacPK antibody, akt antibody, akt1 antibody, dAKT antibody, dAKT/dPKB antibody, dAKT1 antibody, dAkt antibody, dAkt/PKB antibody, dAkt1 antibody, dPKB antibody, dakt antibody, dakt1 antibody, l(3)04226 antibody, l(3)89Bq antibody, p-Akt antibody, pAkt antibody, ACT-5 antibody, akt-1 antibody, pkb antibody, v-akt antibody, v-akt1 antibody, xAct antibody, ATAKT1 antibody, F18A8.2 antibody, F18A8_2 antibody, K+ transporter 1 antibody, POTASSIUM TRANSPORTER antibody, AKT serine/threonine kinase 1 antibody, thymoma viral proto-oncogene 1 antibody, CG4006 gene product from transcript CG4006-RE antibody, actin beta antibody, v-akt murine thymoma viral oncogene homolog 1 S homeolog antibody, v-akt murine thymoma viral oncogene homolog 1 antibody, K+ transporter 1 antibody, Serine/threonine-protein kinase akt-1 antibody, AKT1 antibody, Akt1 antibody, ACTB antibody, akt1.S antibody, akt1 antibody, KT1 antibody, akt-1 antibody
Background
The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far, the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Molecular Weight
60 kDa
Gene ID
207
UniProt
P31749
Pathways
PI3K-Akt Signaling , RTK Signaling , TCR Signaling , AMPK Signaling , Interferon-gamma Pathway , TLR Signaling , Fc-epsilon Receptor Signaling Pathway , EGFR Signaling Pathway , Neurotrophin Signaling Pathway , Response to Water Deprivation , Regulation of Actin Filament Polymerization , Carbohydrate Homeostasis , Glycosaminoglycan Metabolic Process , Cellular Glucan Metabolic Process , Regulation of Muscle Cell Differentiation , Cell-Cell Junction Organization , Regulation of Cell Size , Skeletal Muscle Fiber Development , Regulation of Carbohydrate Metabolic Process , Hepatitis C , Protein targeting to Nucleus , CXCR4-mediated Signaling Events , Signaling Events mediated by VEGFR1 and VEGFR2 , Negative Regulation of intrinsic apoptotic Signaling , Thromboxane A2 Receptor Signaling , Signaling of Hepatocyte Growth Factor Receptor , Positive Regulation of fat Cell Differentiation , VEGFR1 Specific Signals , VEGF Signaling , Warburg Effect
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