Caspase 3 antibody (active, Cleavage Site, N-Term)
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- Target See all Caspase 3 (CASP3) Antibodies
- Caspase 3 (CASP3)
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Binding Specificity
- active, Cleavage Site, N-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Caspase 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- The antibody preferentially recognizes the p17 fragment of the active caspase-3.
- Purification
- Affinity chromatography
- Immunogen
- Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
- Top Product
- Discover our top product CASP3 Primary Antibody
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- Application Notes
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Western Blot: 0.5-4 μg/mL. Immunohistochemistry on paraffin sections: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- PBS containing 50 % glycerol, 1 % BSA, and 0.02 % thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C/-80 °C
- Storage Comment
- Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.
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- Target
- Caspase 3 (CASP3)
- Alternative Name
- Caspase-3 (CASP3 Products)
- Synonyms
- CPP32 antibody, CPP32B antibody, SCA-1 antibody, A830040C14Rik antibody, AC-3 antibody, Apopain antibody, CC3 antibody, Caspase-3 antibody, Lice antibody, Yama antibody, mldy antibody, xcpp32 antibody, casp3 antibody, zgc:100890 antibody, CASP-3 antibody, caspase-3 antibody, caspase 3 antibody, caspase 3 S homeolog antibody, caspase 3, apoptosis-related cysteine peptidase a antibody, caspase 3, apoptosis-related cysteine peptidase antibody, CASP3 antibody, Casp3 antibody, casp3.S antibody, casp3a antibody
- Background
- Caspase-3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). The affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.Synonyms: Apopain, CASP-3, CASP3, CPP-32, CPP32, Cysteine protease CPP32, SCA-1, SCA1, SREBP cleavage activity 1, Yama protein
- Gene ID
- 836
- NCBI Accession
- NP_004337
- UniProt
- P42574
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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