This antibody detects a 55 kDa of glycosylated Fas on SDS-PAGE immunoblots. A 35 kDa band and a 29 KDa of cleaved Fas fragment can also be detected in WB.
Purification
Immunoaffinity Chromatography.
Immunogen
Synthetic peptide corresponding to residues surrounding amino acid 32 of mouse Fas
Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic Synonyms: APT1, Apo-1 antigen, FAS1, FASLG receptor, TNFRSF6, Tumor necrosis factor receptor superfamily member 6