SMAD1 antibody

Catalog No. ABIN127139

Product Details anti-SMAD1 Antibody

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody recognizes ~60 kDa Smad1.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Antigen Affinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acid residues around 253 of human Smad1

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 30 % Glycerol, 0.5 % BSA, and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.

Target Details for SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: SMAD1 (SMAD1 Products)

Synonyms: MLP antibody, Xmad1 antibody, Xsmad1 antibody, bsp1 antibody, jv4-1 antibody, jv41 antibody, madh1 antibody, madr1 antibody, smad-1 antibody, MADH1 antibody, fb39h09 antibody, wu:fb39h09 antibody, wu:fb97e04 antibody, 2/23 antibody, CG12399 antibody, Dmel\\CG12399 antibody, E(zen)2 antibody, En(vvl) antibody, MAD antibody, Mat antibody, P-Mad antibody, P-mad antibody, PMad antibody, Smad antibody, Smad1 antibody, apg antibody, c28 antibody, dMAD antibody, dMad antibody, l(2)K00237 antibody, l(2)k00237 antibody, mad antibody, p-Mad antibody, pMAD antibody, pMad antibody, pSmad antibody, BSP-1 antibody, BSP1 antibody, JV4-1 antibody, JV41 antibody, MADR1 antibody, AI528653 antibody, Madh1 antibody, Madr1 antibody, Mlp1 antibody, MusMLP antibody, smad1 antibody, xmad antibody, SMAD family member 1 antibody, Mothers against dpp antibody, SMAD family member 1 L homeolog antibody, smad1 antibody, SMAD1 antibody, Mad antibody, Smad1 antibody, smad1.L antibody

Background: Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1, Smad5, and Smad8 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF-β and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.Synonyms: BSP-1, BSP1, JV4-1, MAD homolog 1, MADH1, MADR1, Mad-related protein 1, Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, SMAD 1, SMAD family member 1, SMAD-1, Transforming growth factor-beta-signaling protein 1

Gene ID: 4086

NCBI Accession: NP_001003688

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development