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GFP antibody (FITC)

This Mouse Monoclonal antibody specifically detects GFP in WB, ELISA, FM and DB. It exhibits reactivity toward Aequorea victoriaand has been mentioned in 1 publication.
Rockland
Catalog No. ABIN129565
Supplier Product No.: 600-302-215

Quick Overview for GFP antibody (FITC) (ABIN129565)

Target

See all GFP Antibodies
GFP (Green Fluorescent Protein (GFP))

Reactivity

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Aequorea victoria

Host

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Mouse

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Monoclonal

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This GFP antibody is conjugated to FITC

Application

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Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Clone

9F9-F9
  • Supplier Product No.

    600-302-215

    Supplier

    Rockland

    Purpose

    GFP Antibody Fluorescein Conjugated

    Cross-Reactivity (Details)

    Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein and anti-Mouse Serum.

    Characteristics

    Synonyms: mouse anti-GFP antibody FITC conjugation, fluorescein conjugated mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

    Purification

    GFP Fluorescein Conjugated Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography.

    Immunogen

    Immunogen: Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

    Immunogen Type: Recombinant Protein

    Isotype

    IgG1 kappa
  • Application Notes

    Application Note: Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. This antibody was tested by western blotting, for immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

    Western Blot Dilution: 1:10,000-1:25,000

    ELISA Dilution: 1:20,000 - 1:40,000

    IF Microscopy Dilution: 1:500 - 1:2,500

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Expiry Date

    12 months
  • Marks, Nowyhed, Choi, Poholek, Odegard, Flavell, Craft: "Thymic self-reactivity selects natural interleukin 17-producing T cells that can regulate peripheral inflammation." in: Nature immunology, Vol. 10, Issue 10, pp. 1125-32, (2009) (PubMed).

  • Target

    GFP (Green Fluorescent Protein (GFP))

    Alternative Name

    GFP

    Background

    Background: Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

    UniProt

    P42212
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