The antibody Hs-36 reacts with PRKAR2A (protein kinase A regulatory type II alpha subunit), an intra-acrosomal protein.
Cross-Reactivity (Details)
Human
Purification
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Purity
> 95 % (by SDS-PAGE)
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
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Background
Protein kinase cAMP-dependent type II regulatory s,PRKAR2A (proteinkinase A regulatory type II alpha subunit), also known as PKR2, or PRKAR2, is a component of cAMP-dependent protein kinase complex. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The PRKAR2A subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). In sperm, this antigen can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,PKAR2A, PKR2, PKAR2, proteinkinase A regulatory 2