CD43 antibody (Biotin)

Catalog No. ABIN133167

Product Details anti-CD43 Antibody (Biotin)

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: Rat CD43

Immunogen: Rat thymocyte membrane . Fusion Partner: NS1/1 . Immunocyte Donor: BALB/c spleen

Clone: W3-13HLK

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/ml

Buffer: Biotin conjugated Ig buffered in PBS, 0.02% NaN and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43 (Leukosialin) (SPN Products)

Synonyms: CD43 antibody, GALGP antibody, GPL115 antibody, LSN antibody, A630014B01Rik antibody, Cd43 antibody, Galgp antibody, Ly-48 antibody, Ly48 antibody, Lsn antibody, Lsn1 antibody, gpL115 antibody, sialophorin antibody, SPN antibody, Spn antibody

Background: Anti-rat CD43 monoclonal antibody recognizes a monomorphic determinant expressed on rat thymocytes, polymorphonuclear cells, plasma cells and stem cells, but not B lymphocytes or pre-B cells (1,3). The antigen is a heavily glycosylated glycoprotein of apparent molecular weight 95 kDa and has a high content of O-linked carbohydrate structures (3). This major glycoprotein of thymocytes and T lymphocytes is referred to by several names including leukocyte sialoglycoprotein and leukosialin. The carbohydrate structures of leukosialin account for approximately 60% of its weight (2). On thymocytes, this glycoprotein is the main target for binding of peanut lectin (4). This antibody is useful for labelling T but not B lymphocytes and in studies on stem cells since pre-B cells are not labelled while the multipotential stem cell is. It may also be used in analysis of NK cells (5) and in molecular studies of the sialoglycoprotein which it recognizes.

Pathways: Regulation of Leukocyte Mediated Immunity