CD8 alpha antibody (Biotin)

Catalog No. ABIN133186

Product Details anti-CD8 alpha Antibody (Biotin)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: Rat CD8a

Immunogen: High molecular weight rat thymocyte glycoproteins . Fusion Partner: P3-NSI-1-Ag4 (NSI/1) . Donor: BALB/c spleen

Clone: OX-8

Isotype: IgG1

Application Details

Application Notes: This clone is reported to work with frozen and paraffin sections (9).

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/ml

Buffer: Biotin conjugated Ig buffered in PBS, 0.02% NaN and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a (CD8A Products)

Synonyms: cd8 antibody, LOC100136450 antibody, BB154331 antibody, Ly-2 antibody, Ly-35 antibody, Ly-B antibody, Lyt-2 antibody, zgc:136643 antibody, CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, RHACD8-4 antibody, CD8a molecule antibody, uncharacterized LOC100125537 antibody, CD8 alpha antibody, CD8 antigen, alpha chain antibody, T-cell surface glycoprotein CD8 alpha chain antibody, Cd8a antibody, LOC100125537 antibody, LOC100136450 antibody, CD8A antibody, cd8a antibody, LOC100126556 antibody

Background: Anti-rat CD8a monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells, the majority of NK cells, and the granular intraepithelial leukocytes in the small intestine. (1,2,3,4.) The antigen recognized is a complex of surface glycoproteins of Mr 34,000, 39,000 and 76,000 Da and is the rat homologue of the human CD8 and the mouse Ly-2,3 antigen. (5,8) ABIN133186labels all peripheral T cells that are unlabelled by the ABIN133186(W3/25) monoclonal antibody. It labels a T-cell subset which mediates the suppression of antibody formation (1) and the cytotoxic cell precursor. (2) ABIN133186and ABIN133186can be used together to fractionate T cells by sorting in FACS or by rosette depletion (1,2) or can be used together to study subsets of T cells in the rat which mediate lethal graft vs. host disease. (7) This antibody is one of the 3 monoclonal antibodies which labels T lymphocyte populations in the rat, these being ABIN133186(W3/13) which labels all T cells, as well as ABIN133186(W3/25) and CL004F` (MRC 0X-8) which label non-overlapping T cell populations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (9).

Pathways: TCR Signaling