RT1.D antibody (Biotin)
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- Target
- RT1.D
- Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Biotin
- Application
- Flow Cytometry (FACS)
- Specificity
- Rat RT1.D (Rat Ia-E)
- Immunogen
- Rat spleen membrane glycoproteins depleted of Ia-A antigens. . Fusion Partner: X63 Ag8.653 . Immunocyte Donor: BALB/c spleen
- Clone
- OX-17
- Isotype
- IgG1
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- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/ml
- Buffer
- Biotin conjugated Ig buffered in PBS, 0.02% NaN and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography)
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
-
- Target
- RT1.D
- Background
- Anti-rat RT1.D monoclonal antibody recognizes a monomorphic determinant on the chain of the rat Ia antigen and appears to be the rat homologue of mouse Ia-E. It recognizes the rat Ia product present on B, but not T cells from lymph node or thoracic duct lymph. It does not bind to thymocytes or erythrocytes. The antibody does not cross-react with rat Ia-A or mouse Ia-E antigen, but rabbit antibody raised against the antibody affinity column-purified MRC OX-17 antigen cross-reacted on tissues of mice expressing Ia-E mouse antigen but not on those mouse strains that were Ia-E antigen negative (2).
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