RT1.D antibody (PE)

Catalog No. ABIN133249

Product Details

Target: RT1.D

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: PE

Application: Flow Cytometry (FACS)

Specificity: Rat RT1.D (Rat Ia-E)

Purification: Purified from ascitic fluid via Protein G Chromatography.

Immunogen: Rat spleen membrane glycoproteins depleted of Ia-A antigens. . Fusion Partner: X63 Ag8.653 . Immunocyte Donor: BALB/c spleen

Clone: OX-17

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/ml

Buffer: R-PE conjugated Ig buffered in PBS, 0.02% NaN and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: RT1.D

Background: Anti-rat RT1.D monoclonal antibody recognizes a monomorphic determinant on the chain of the rat Ia antigen and appears to be the rat homologue of mouse Ia-E. It recognizes the rat Ia product present on B, but not T cells from lymph node or thoracic duct lymph. It does not bind to thymocytes or erythrocytes. The antibody does not cross-react with rat Ia-A or mouse Ia-E antigen, but rabbit antibody raised against the antibody affinity column-purified MRC OX-17 antigen cross-reacted on tissues of mice expressing Ia-E mouse antigen but not on those mouse strains that were Ia-E antigen negative (2).