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PKM2 antibody (AA 405-409)
PKM2
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-PKM2 Antibody
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Target
See all PKM2 Antibodies
PKM2
(Pyruvate Kinase M2 (PKM2))
Binding Specificity
All epitopes for PKM2 antibodies
AA 405-409
Reactivity
All reactivities for PKM2 antibodies
Human
Host
All hosts for PKM2 antibodies
Rabbit
Clonality
All clonalities for PKM2 antibodies
Polyclonal
Conjugate
All conjugates for PKM2 antibodies
This PKM2 antibody is un-conjugated
Application
All applications for PKM2 antibodies
Western Blotting (WB)
Specificity
The antibody detects endogenous levels of total PKM2 protein.
Immunogen
Peptide sequence around AA 405-409 (T-S-D-P-T) derived from Human PKM2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
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Alternatives
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Application Details
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Application Notes
Western blotting: 1:500-1:1000
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
References for anti-PKM2 antibody (ABIN1355885)
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Jiang, Li, Yang, Hawke, Zheng, Xia, Aldape, Wei, Guo, Chen, Lu : "PKM2 regulates chromosome segregation and mitosis progression of tumor cells. " in: Molecular cell , Vol. 53 , Issue 1 , pp. 75-87 , (2014 ) (PubMed ).
Yang, Xia, Hawke, Li, Liang, Xing, Aldape, Hunter, Alfred Yung, Lu : "PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis. " in: Cell , Vol. 150 , Issue 4 , pp. 685-96 , (2012 ) (PubMed ).
Yang, Zheng, Xia, Ji, Chen, Guo, Lyssiotis, Aldape, Cantley, Lu : "ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect. " in: Nature cell biology , Vol. 14 , Issue 12 , pp. 1295-304 , (2012 ) (PubMed ).
Target Details for PKM2
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Target
PKM2
(Pyruvate Kinase M2 (PKM2))
Alternative Name
PKM2 (PKM2 Products )
Synonyms
CTHBP antibody, OIP3 antibody, PK3 antibody, PKM2 antibody, TCB antibody, THBP1 antibody, AA414905 antibody, AL024370 antibody, AL024424 antibody, Pk-2 antibody, Pk-3 antibody, Pk3 antibody, Pkm2 antibody, PKM12 antibody, PKM antibody, pyruvate kinase M1/2 antibody, pyruvate kinase, muscle antibody, PKM antibody, Pkm antibody
Background
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Molecular Weight
60 kDa
NCBI Accession
NP_872270
Pathways
Warburg Effect
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