CD8 alpha antibody

Catalog No. ABIN135590

Product Details anti-CD8 alpha Antibody

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Chicken CD8α, Mr 34 kDa

Characteristics: Mouse Anti-Chicken CD8a-UNLB

Purification: Purified

Immunogen: Chicken splenocytes

Clone: EP72

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a (CD8A Products)

Synonyms: cd8 antibody, LOC100136450 antibody, BB154331 antibody, Ly-2 antibody, Ly-35 antibody, Ly-B antibody, Lyt-2 antibody, zgc:136643 antibody, CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, RHACD8-4 antibody, CD8a molecule antibody, uncharacterized LOC100125537 antibody, CD8 alpha antibody, CD8 antigen, alpha chain antibody, T-cell surface glycoprotein CD8 alpha chain antibody, Cd8a antibody, LOC100125537 antibody, LOC100136450 antibody, CD8A antibody, cd8a antibody, LOC100126556 antibody

Background: In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two chains, and (ii) a heterodimer of an chain and a chain. Chicken CD8 is expressed on approximately 80 % of thymocytes, 45 % of blood mononuclear cells and 50 % of spleen cells, but < 1 % of cells in the bursa and bone marrow. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8- and CD8-chains, a relatively large proportion of the CD8+ TCR- cells in the spleens of embryos and young chicks express only the -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- CD8 cells in the chicken that is not found in rodents.

Pathways: TCR Signaling