Nitrotyrosine antibody (HRP)
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- Target See all Nitrotyrosine Antibodies
- Nitrotyrosine
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Nitrotyrosine antibody is conjugated to HRP
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Human, Mouse, Rat
- Cross-Reactivity (Details)
- Nitrotyrosine
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated to Nitrotyrosine
- Isotype
- IgG
- Top Product
- Discover our top product Nitrotyrosine Primary Antibody
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- Application Notes
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WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Nitrotyrosine
- Abstract
- Nitrotyrosine Products
- Target Type
- Chemical
- Background
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Synonyms: NULL
Background: Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques and chronic renal failure.
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