MARCH10 antibody (HRP)

Catalog No. ABIN1419562

Product Details anti-MARCH10 Antibody (HRP)

Target: MARCH10 (Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH10 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RNF190

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MARCH10

Target: MARCH10 (Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10))

Alternative Name: RNF190 (MARCH10 Products)

Synonyms: 4933417C16Rik antibody, AV039223 antibody, OTTMUSG00000002847 antibody, Rnf190 antibody, MARCH-X antibody, RGD1311692 antibody, RNF190 antibody, membrane associated ring-CH-type finger 10 antibody, membrane-associated ring finger (C3HC4) 10 antibody, MARCH10 antibody, March10 antibody

Background:

Synonyms: MARCH 10, MARCH X, MARCH-X, MARCH10, MARHA_HUMAN, Membrane associated ring finger C3HC4 10, Membrane associated ring finger C3HC4 10, E3 ubiquitin protein ligase, Membrane associated ring finger 10, Membrane associated RING finger protein 10, Membrane-associated RING finger protein 10, Membrane-associated RING-CH protein X, Probable E3 ubiquitin protein ligase MARCH10, Probable E3 ubiquitin-protein ligase MARCH10, Ring finger protein 190, RNF 190, RNF190.

Background: The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. MARCH10 (membrane-associated ring finger (C3HC4) 10), also known as RNF190 (ring finger protein 190) or MARCH-X, is an 808 amino acid protein with one RING-CH-type zinc finger domain. MARCH10 may function as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfers that residue to a protein that is targeted for degradation.

Gene ID: 162333