CHAF1A antibody (HRP)

Catalog No. ABIN1420318

Product Details anti-CHAF1A Antibody (HRP)

Target: CHAF1A (Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHAF1A antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human p150 CAF1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CHAF1A

Target: CHAF1A (Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))

Alternative Name: p150 CAF1 (CHAF1A Products)

Synonyms: CHAF1A antibody, CAF-1 antibody, CAF1 antibody, CAF1B antibody, CAF1P150 antibody, P150 antibody, AL023013 antibody, AL024058 antibody, Cac1p antibody, CAF-1P150 antibody, CAF-I-p150 antibody, CAF-Ip150 antibody, caip150 antibody, wu:fd14g10 antibody, zgc:153930 antibody, chaf1a-b antibody, xp150 antibody, chromatin assembly factor 1 subunit A antibody, chromatin assembly factor 1, subunit A (p150) antibody, chromatin assembly factor 1 subunit A S homeolog antibody, Chaf1a antibody, CHAF1A antibody, LOC100447657 antibody, chaf1a antibody, chaf1a.S antibody

Background:

Synonyms: CAF-1 p150, CAF 1 150 kDa subunit, CAF 1, CAF 1 subunit A, CAF, CAF I 150 kDa subunit, CAF I p150, CAF Ip150, CAF-1 subunit A, CAF-I 150 kDa subunit, CAF-I p150, CAF1 150 kDa subunit, CAF1, CAF1 p150 Subunit, CAF1 subunit A, CAF1A, CAF1A, CAF1A_HUMAN, CAF1B, CAF1P150, CAF1P155, CAF1P155, CHAF1A, Chromatin assembly factor 1 subunit A, Chromatin Assembly Factor 1 Subunit A p150, Chromatin assembly factor I 150 kDa, Chromatin assembly factor I 150 kDa, Chromatin Assembly Factor I, Chromatin assembly factor I p150 subunit, DCAF1, hp150, Nucleosome Remodeling Factor 150 kDa Subunit, NURF150, P150.

Background: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA, histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Gene ID: 10036