PIK3CA antibody (AA 470-519)

Catalog No. ABIN1453773

The Rabbit Polyclonal anti-PIK3CA antibody (ABIN1453773) specifically detects PIK3CA in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PIK3CA antibody (AA 470-519) (ABIN1453773)

Target: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3CA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PIK3CA Antibody

Binding Specificity: AA 470-519

Purpose: PI 3-kinase p110α Antibody

Specificity: PI 3-kinase p110α Polyclonal Antibody detects endogenous levels of PI 3-kinase p110α protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from human PI 3-kinase p110alpha. AA range:470-519

Isotype: IgG

Application Details

Application Notes: IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PIK3CA

Target: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Alternative Name: PI 3-kinase p110Alpha

Background:

Synonyms: PIK3CA, Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit

Background: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit.The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016],

Gene Name: PIK3CA

Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

Molecular Weight: 124 kDa

Gene ID: 5290

OMIM: 171834

UniProt: P42336

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling