SATB1 antibody (AA 668-681)

Catalog No. ABIN1493900

Product Details anti-SATB1 Antibody

Target: SATB1 (SATB Homeobox 1 (SATB1))

Binding Specificity: AA 668-681

Reactivity: Human, Mouse, Rat, Rabbit, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This SATB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SATB1. Reported variants NP_001124482.1 and NP_002962.1 represent identical protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Rabbit (100%) Elephant, Opossum, Lizard (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-ESDEENRQKTRPRT from an internal region of human SATB1 (NP_002962.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Rabbit (100%), Elephant, Opossum, Lizard (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Experiments gave bands at approx. 110 kDa and 85 kDa in lysates of cell line MOLT4 after 0.3 μg/mL antibody staining. The observed 110 kDa band corresponds to earlier findings in literature with different antibodies (De Belle et al, J Cell Biol. 1998 Apr 20,141(2):335-48., PMID: 9548713). This protein has a calculated MW of 86.0 kDa according to NP_002962.1. Recommended concentration: 0.3-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SATB1

Target: SATB1 (SATB Homeobox 1 (SATB1))

Alternative Name: SATB1 (SATB1 Products)

Synonyms: 2610306G12Rik antibody, AW413156 antibody, SATB1 antibody, sb:cb955 antibody, si:ch211-195k18.2 antibody, SATB homeobox 1 antibody, special AT-rich sequence binding protein 1 antibody, SATB homeobox 1a antibody, SATB1 antibody, Satb1 antibody, satb1 antibody, satb1a antibody

Background: Name/Gene ID: SATB1

Synonyms: SATB1, DNA-binding protein SATB1, SATB homeobox 1

Gene ID: 6304

NCBI Accession: NP_002962

UniProt: Q01826

Pathways: Caspase Cascade in Apoptosis, Activated T Cell Proliferation