KEAP1 antibody (AA 41-53)

Catalog No. ABIN1494037

Product Details anti-KEAP1 Antibody

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Binding Specificity: AA 41-53

Reactivity: Human, Pig, Cow, Dog, Horse, Monkey, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human KEAP1. Reported variants represent identical protein: NP_036421.2, NP_987096.1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum (100%) Mouse, Rat, Hamster (92%) Lizard (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EVTPSQHGNRTFS from an internal region (near N-terminus) of human KEAP1 (NP_036421.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum (100%), Mouse, Rat, Hamster (92%), Lizard (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.2 - 0.6 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75kD band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 69.7kD according to Human NP_036421.2 and of 69.6kD according to Mouse NP_057888.1). Recommended concentration: 0.2-0.6 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1 (KEAP1 Products)

Synonyms: INrf2 antibody, KLHL19 antibody, INRF2 antibody, mKIAA0132 antibody, Inrf2 antibody, fb36f11 antibody, keap1 antibody, wu:fb36f11 antibody, KEAP1 antibody, klhl19 antibody, zgc:171654 antibody, kelch like ECH associated protein 1 antibody, kelch-like ECH-associated protein 1 antibody, Kelch-like ECH-associated protein 1 antibody, kelch-like ECH-associated protein 1a antibody, kelch like ECH associated protein 1 L homeolog antibody, kelch-like ECH-associated protein 1b antibody, KEAP1 antibody, Keap1 antibody, keap1a antibody, keap1 antibody, keap1.L antibody, keap1b antibody

Background: Name/Gene ID: KEAP1

Synonyms: KEAP1, Cytosolic inhibitor of Nrf2, INrf2, KLHL19, Kelch-like protein 19, KIAA0132

Gene ID: 9817

NCBI Accession: NP_036421

UniProt: Q14145

Pathways: Maintenance of Protein Location