NCKAP1 antibody (N-Term)

Catalog No. ABIN1494055

Product Details anti-NCKAP1 Antibody

Target: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCKAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Human NCKAP1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse (98%) Rat (97%) Xenopus (94%) Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 2 and 338 of NCKAP1 (SwissProt Q9Y2A7). Percent identity by BLAST analysis: Human (100%), Mouse (98%), Rat (97%), Xenopus (94%), Zebrafish (92%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7, 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for NCKAP1

Target: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Alternative Name: NCKAP1 / NAP125 (NCKAP1 Products)

Synonyms: HEM2 antibody, NAP1 antibody, NAP125 antibody, p125Nap1 antibody, C79304 antibody, H19 antibody, Hem-2 antibody, Hem2 antibody, Nap1 antibody, mKIAA0587 antibody, mh19 antibody, NCK associated protein 1 antibody, NCK-associated protein 1 antibody, NCK-associated protein 1 L homeolog antibody, NCKAP1 antibody, Nckap1 antibody, nckap1.L antibody

Background: Name/Gene ID: NCKAP1

Synonyms: NCKAP1, HEM2, KIAA0587, NAP1, NAP125, p125Nap1, NAP 1, NCK-associated protein 1

Gene ID: 10787

UniProt: Q9Y2A7

Pathways: RTK Signaling, Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis