COL18A1 antibody

Catalog No. ABIN1498024

Product Details anti-COL18A1 Antibody

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL18A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human Endostatin

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Endostatin.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B8215 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. ELISA: To detect hEndostatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Endostatin (LS-C104550) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEndostatin. Western Blot: To detect hEndostatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEndostatin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for COL18A1

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Alternative Name: COL18A1 / Endostatin (COL18A1 Products)

Synonyms: KNO antibody, KNO1 antibody, KS antibody, cb371 antibody, cb529 antibody, fc96d06 antibody, sb:cb529 antibody, wu:fb47h10 antibody, wu:fb77g12 antibody, wu:fc50f04 antibody, wu:fc96d06 antibody, wu:fk02h12 antibody, kno antibody, kno1 antibody, collagen type XVIII alpha 1 chain antibody, collagen type XVIII alpha 1 chain a antibody, collagen, type XVIII, alpha 1 antibody, collagen type XVIII alpha 1 antibody, COL18A1 antibody, col18a1a antibody, Col18a1 antibody, col18a1 antibody

Target Type: Chemical

Background: Name/Gene ID: COL18A1
Family: Collagen

Synonyms: COL18A1, Collagen, type XVIII, alpha 1, Collagen alpha-1(XVIII) chain, Endostatin, KNO, KNO1, KS, Multi-functional protein MFP, Antiangiogenic agent, Collagen alpha 1(xviii), Knobloch syndrome, type 1

Gene ID: 80781

UniProt: P39060