Insulin Receptor antibody (pTyr1355)

Catalog No. ABIN1531327

The Rabbit Polyclonal anti-Insulin Receptor antibody is suitable to detect Insulin Receptor in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Insulin Receptor antibody (pTyr1355) (ABIN1531327)

Target: Insulin Receptor (INSR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Insulin Receptor Antibody

Binding Specificity: AA 1326-1375, pTyr1355

Purpose: Insulin R (phospho Tyr1355) Antibody

Specificity: Phospho-Insulin R (Y1355) Polyclonal Antibody detects endogenous levels of Insulin R protein only when phosphorylated at Y1355.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human IR around the phosphorylation site of Tyr1355. AA range:1326-1375

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Background:

Synonyms: INSR, Insulin receptor, IR, CD antigen CD220

Background: This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015],

Gene Name: INSR

Protein Name: Insulin receptor

Molecular Weight: 156 kDa

Gene ID: 3643

OMIM: 125853

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity