FOXO3 antibody (pSer253)

Catalog No. ABIN1531845

The Rabbit Polyclonal anti-FOXO3 antibody (ABIN1531845) specifically detects FOXO3 in WB, IHC and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for FOXO3 antibody (pSer253) (ABIN1531845)

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-FOXO3 Antibody

Binding Specificity: AA 220-269, pSer253

Specificity:

FKHRL1 (Phospho-Ser253) Antibody detects endogenous levels of FKHRL1 only when phosphorylated at Ser253.

PhosphorylationH:S253 M:S252

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human FKHRL1 around the phosphorylation site of Ser253.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.220950 (NCBI Gene Symbol: FOXO3)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FKHRL1

Background:

Synonyms: AF6q21 protein, FKHR2, FKHRL1, Forkhead box O3, forkhead box O3A, Forkhead box protein O3A, Forkhead in rhabdomyosarcoma-like 1, FOXO3

NCBI Gene Symbol: FOXO3

Molecular Weight: 71 kDa

Gene ID: 2309

OMIM: 602681

UniProt: O43524

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis