c-MYC antibody (pThr58)

Catalog No. ABIN1531913

The Rabbit Polyclonal anti-c-MYC antibody has been validated for WB, IHC, ELISA and IP. It is suitable to detect c-MYC in samples from Human, Mouse and Rat.

Quick Overview for c-MYC antibody (pThr58) (ABIN1531913)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-MYC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-c-MYC Antibody

Binding Specificity: AA 25-74, pThr58

Specificity:

Myc (Phospho-Thr58) Antibody detects endogenous levels of Myc only when phosphorylated at Thr58.

PhosphorylationH:T58 M:T58 R:T58

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Myc around the phosphorylation site of Thr58.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.202453 (NCBI Gene Symbol: MYC)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: Myc Proto-Oncogene protein

Background:

Synonyms: Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64 , MYC , BHLHE39

NCBI Gene Symbol: MYC

Molecular Weight: 48 kDa

Gene ID: 9531

OMIM: 190080

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect