PCNA antibody (AA 61-110)

Catalog No. ABIN1533376

The Rabbit Polyclonal anti-PCNA antibody is suitable to detect PCNA in samples from Human, Mouse, Rat, Monkey and Rabbit. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for PCNA antibody (AA 61-110) (ABIN1533376)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Monkey, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PCNA Antibody

Binding Specificity: AA 61-110

Purpose: PCNA Antibody

Specificity: PCNA Polyclonal Antibody detects endogenous levels of PCNA protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PCNA. AA range:61-110

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:100-500,IF ICC 1:100-500,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background:

Synonyms: PCNA, Proliferating cell nuclear antigen, PCNA, Cyclin

Background: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],

Gene Name: PCNA

Protein Name: Proliferating cell nuclear antigen

Molecular Weight: 28 kDa

Gene ID: 5111

OMIM: 176740

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy