Presenilin 1 antibody (N-Term)

Catalog No. ABIN1573952

Product Details anti-Presenilin 1 Antibody

Target: Presenilin 1 (PSEN1)

Binding Specificity: AA 25-50, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Presenilin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Rabbit Anti-Presenilin 1 Polyclonal Antibody can detect endogenous levels of the full-length protein (approx. 43-52 kD) and the N-terminal fragment of presenilin 1 (~ 28 kD).

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding - AA 25-50 (TVRSQNDNRERQEHNDRRSLGHPEPL) of the N-terminus of human presenilin 1, which is exposed - the extracellular region, KLH conjugated.

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-2.0 µg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 30 % glycerol, and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-Presenilin 1 antibody (ABIN1573952)

Puri, Yerrathota, Home, Idowu, Chakravarthi, Ward, Singhal, Vanden Heuvel, Fields, Sharma: "Notch4 activation aggravates NF-κB-mediated inflammation in HIV-1-associated nephropathy." in: Disease models & mechanisms, Vol. 12, Issue 12, (2020) (PubMed).

Target Details for Presenilin 1

Target: Presenilin 1 (PSEN1)

Alternative Name: Presenilin 1 (PSEN1 Products)

Synonyms: pre1 antibody, psen antibody, zfPS1 antibody, zf-PS1 antibody, ad3 antibody, fad antibody, ps1 antibody, s182 antibody, X-PS-beta antibody, X-PS-alpha antibody, AD3 antibody, FAD antibody, PS-1 antibody, PS1 antibody, S182 antibody, Ad3h antibody, presenilin 1 antibody, presenilin 1 L homeolog antibody, psen1 antibody, PSEN1 antibody, Psen1 antibody, psen1.L antibody

Background: Presenilin 1 (PS1) and presenilin 2 (PS2) are catalytic subunits of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). The other members of the gamma-secretase complex are required to have protease activity. PS1 and PS2 may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It regulates epithelial-cadherin function. Three causative genes have been identified that when mutated lead to presenile Alzheimer's disease: APP (amyloid precursor protein gene), PSEN1, and PSEN2. The presenilin 1 gene has recently been discovered on chromosome 14 and is responsible for an early onset form of Alzheimer's disease that accounts for 70-80% of the inherited forms of Alzheimer's. It is a membrane protein that has a region where at least seven mutations have been identified in family members with Alzheimer's disease. Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (28 kD) and carboxy-terminal (~17 kD) fragments.Rabbit Anti-Presenilin 1 Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide, TVRSQNDNRERQEHNDRRSLGHPEPL, corresponding to amino acids 25-50 of the N-terminus of human presenilin 1.Rabbit Anti-Presenilin 1 Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 0.5 mg/ml.

Pathways: Notch Signaling, EGFR Signaling Pathway, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport