ATP6V1A antibody

Catalog No. ABIN1573979

Product Details anti-ATP6V1A Antibody

Target: ATP6V1A (ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A (ATP6V1A))

Reactivity: Human, Mouse, Cow, Chicken, Drosophila melanogaster, Fish, Manduca, Nicotiana tabacum, Orang-Utan

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody reacts with mouse V-ATPase subunit A. Sequence homology predicts that it will also react with human, bovine, orangutan, tobacco hornworm, chicken, fruit fly, and zebrafish V-ATPase subunit A.

Cross-Reactivity (Details): Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody reacts with mouse V-ATPase subunit A. Sequence homology predicts that it will also react with human, bovine, orangutan, tobacco hornworm, chicken, fruit fly, and zebrafish V-ATPase subunit A.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide conjugated - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.02-0.2 µg/mL
Western blot: 1.0 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 30 % glycerol, and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8 °C. For long term storage, aliquot and store at -28 °C or below.

References for anti-ATP6V1A antibody (ABIN1573979)

Gokey, Dasgupta, Amack: "The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish." in: Developmental biology, Vol. 407, Issue 1, pp. 115-30, (2016) (PubMed).

Grubman, Lidgerwood, Duncan, Bica, Tan, Parker, Caragounis, Meyerowitz, Volitakis, Moujalled, Liddell, Hickey, Horne, Longmuir, Koistinaho, Donnelly, Crouch, Tammen, White, Kanninen: "Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder." in: Acta neuropathologica communications, Vol. 2, pp. 25, (2014) (PubMed).

Monteiro, Aires, Becker, Jacinto, Certal, Rodríguez-León: "V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration." in: PLoS ONE, Vol. 9, Issue 3, pp. e92594, (2014) (PubMed).

Li, Chen, Klemmer, Koopmans, Karupothula, Smit: "Identifying true protein complex constituents in interaction proteomics: the example of the DMXL2 protein complex." in: Proteomics, Vol. 12, Issue 15-16, pp. 2428-32, (2012) (PubMed).

Nylandsted, Becker, Bunkenborg, Andersen, Dengjel, Jäättelä: "ErbB2-associated changes in the lysosomal proteome." in: Proteomics, Vol. 11, Issue 14, pp. 2830-8, (2011) (PubMed).

Target Details for ATP6V1A

Target: ATP6V1A (ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A (ATP6V1A))

Alternative Name: V-ATPase Subunit A (ATP6V1A Products)

Synonyms: ATP6A1 antibody, ATP6V1A1 antibody, HO68 antibody, VA68 antibody, VPP2 antibody, Vma1 antibody, AI647066 antibody, Atp6a1 antibody, Atp6a2 antibody, Atp6v1a1 antibody, ATP6V1A antibody, atp6v1al antibody, zgc:63516 antibody, An02g10440 antibody, AO090102000349 antibody, vacuolar ATP synthase subunit A antibody, atp6a1 antibody, atp6v1a1 antibody, ho68 antibody, v1a antibody, va68 antibody, vma1 antibody, vpp2 antibody, CG12403 antibody, Dmel\CG12403 antibody, V-ATPase antibody, Vha antibody, Vha-68-1 antibody, Vha68 antibody, vha67-2 antibody, vha68-1 antibody, ATPase H+ transporting V1 subunit A antibody, ATPase, H+ transporting, lysosomal V1 subunit A antibody, ATPase, H+ transporting, lysosomal, V1 subunit Aa antibody, v-type proton ATPase catalytic subunit A antibody, vacuolar ATP synthase subunit A antibody, ATPase H+ transporting V1 subunit A L homeolog antibody, vacuolar proton pump 3 antibody, Vacuolar H[+] ATPase 68kD subunit 1 antibody, ATP6V1A antibody, Atp6v1a antibody, atp6v1aa antibody, ANI_1_1468024 antibody, AOR_1_602134 antibody, VHA-A antibody, atp6v1a.L antibody, vpp3 antibody, Vha68-1 antibody

Background: The V-ATPase subunit A is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide, purified from rabbit antiserum by immunoaffinity chromatography, and supplied as 40 ug aliquot at concentration of 0.5 mg/ml.

Pathways: Transition Metal Ion Homeostasis, Proton Transport, SARS-CoV-2 Protein Interactome