PRKAG2 antibody (C-Term)

Catalog No. ABIN1574210

Product Details anti-PRKAG2 Antibody

Target: PRKAG2 (Protein Kinase, AMP-Activated, gamma 2 Non-Catalytic Subunit (PRKAG2))

Binding Specificity: C-Term

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAG2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: AMPKgamma2 Antibody, pAb, Rabbit detects human endogenous AMPKgamma2 and does not cross-react with AMPKgamma1 and AMPKgamma3. It is predicted to react with mouse and rat AMPKgamma2 according to sequence homology.

Cross-Reactivity (Details): AMPKγ2 Antibody, pAb, Rabbit detects human endogenous AMPKγ2 and does not cross-react with AMPKγ1 and AMPKγ3. It is predicted to react with mouse and rat AMPKγ2 according to sequence homology.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide from C-terminal of human AMPKγ2

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mL Other Applications: User optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for PRKAG2

Target: PRKAG2 (Protein Kinase, AMP-Activated, gamma 2 Non-Catalytic Subunit (PRKAG2))

Alternative Name: AMPKgamma2 (PRKAG2 Products)

Synonyms: MGC82938 antibody, PRKAG2 antibody, si:ch211-230l11.1 antibody, si:dkey-217f4.2 antibody, AAKG antibody, AAKG2 antibody, CMH6 antibody, H91620p antibody, WPWS antibody, 2410051C13Rik antibody, AI854673 antibody, protein kinase AMP-activated non-catalytic subunit gamma 2 antibody, protein kinase, AMP-activated, gamma 2 non-catalytic subunit L homeolog antibody, 5'-AMP-activated protein kinase subunit gamma-2 antibody, protein kinase, AMP-activated, gamma 2 non-catalytic subunit b antibody, protein kinase, AMP-activated, gamma 2 non-catalytic subunit antibody, Prkag2 antibody, PRKAG2 antibody, prkag2.L antibody, LOC504219 antibody, prkag2b antibody, prkag2 antibody

Background: AMP-activated protein kinase (AMPK) plays an important role in the regulation of energy homeostasis. It is activated by an elevated AMP/ATP ratio caused by cellular and environmental stress, such as heat shock, hypoxia, and ischemia. AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits, each of which is encoded by two or three distinct genes (alpha1, alpha2, beta1, beta2, gamma1, gamma2, gamma3). AMPKgamma2 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human AMPKgamma2 (Swiss Prot: Q9UGJ0).

Pathways: AMPK Signaling, Cellular Glucan Metabolic Process, Ribonucleoside Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Warburg Effect