ARG antibody (Biotin)

Catalog No. ABIN1607772

Product Details anti-ARG Antibody (Biotin)

Target: ARG (Arginase (ARG))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARG antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Arginase from other sources is unknown.

Purity: Anti-Arginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Arginase [Bovine Liver].

Endotoxin Level: Low Endotoxin : No

Immunogen: Arginase [Bovine Liver]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Arginase has been assayed against 1.0 µg of Arginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1.000 to 1:4.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for ARG

Target: ARG (Arginase (ARG))

Alternative Name: Arginase (ARG Products)

Synonyms: CG18104 antibody, Dmel\\CG18104 antibody, EG:171D11.4 antibody, EG:65F1.3 antibody, SI:zC146F4.4 (novel protein with NUDIX domain) antibody, si:ch211-146f4.3 antibody, Tb08.26N11.490 antibody, rocF antibody, NV10213 antibody, argi1 antibody, arginase antibody, arginase 1 L homeolog antibody, arginase 1 antibody, Arginase-1 antibody, arg antibody, arg1.L antibody, arg1 antibody, ARGAH1 antibody, BP0538 antibody, rocF antibody, SAS2066 antibody, RR_RS05730 antibody, CND03500 antibody, CNG00550 antibody, Tb927.8.2020 antibody, PGTG_16455 antibody, LOC100123155 antibody, argi1 antibody

Background: Anti-Arginase antibody is localized in the cytoplasm and plays a key role in the Arginine Metabolism Urea Cycle. Anti-Arginase antibody, belonging to the arginase family, is ideal for researchers involved in Metabolism, Cancer, and Signal Transduction research. Synonyms: A I antibody, Al antibody, ARG 1 antibody, ARG1 antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase1 antibody, Liver type arginase antibody, Type I arginase antibody

Gene ID: 513608

UniProt: Q2KJ64