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Fructosyl Amino Acid Oxidase antibody (Biotin)

socD Reactivity: Corynebacterium WB, ELISA, IP Host: Sheep Polyclonal Biotin
Catalog No. ABIN1607846
  • Target See all Fructosyl Amino Acid Oxidase (socD) products
    Fructosyl Amino Acid Oxidase (socD)
    Reactivity
    • 4
    • 2
    • 1
    Corynebacterium
    Host
    • 6
    • 1
    Sheep
    Clonality
    • 7
    Polyclonal
    Conjugate
    • 4
    • 2
    • 1
    This Fructosyl Amino Acid Oxidase antibody is conjugated to Biotin
    Application
    • 7
    • 6
    • 3
    • 3
    • 2
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Cross-Reactivity (Details)
    Cross reactivity against Fructosyl Amino Acid Oxidase from other sources is unknown.
    Purity
    Anti-Fructosyl-Amino Acid Oxidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Fructosyl Amino Acid Oxidase [E.coli].
    Endotoxin Level
    Low Endotoxin : No
    Immunogen
    Fructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli]
    Immunogen Type: Recombinant Protein
    Isotype
    IgG
  • Application Notes
    Anti-Fructosyl-Amino Acid Oxidase Antibody has been assayed against 1.0 µg of Fructosyl Amino Acid Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:8.000 to 1:34.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Handling Advice
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Expiry Date
    Expiration date is one (1) year from date of opening.
  • Target
    Fructosyl Amino Acid Oxidase (socD)
    Alternative Name
    Fructosyl-Amino Acid Oxidase (socD Products)
    Synonyms
    fructosyl amino acid oxidase antibody, hypothetical protein antibody, socD antibody, CNB00220 antibody, AFUA_8G06440 antibody, AFUA_6G03440 antibody, PTRG_01708 antibody, ANI_1_1464184 antibody, AOR_1_60064 antibody, PGTG_09157 antibody
    Background
    Anti-Fructosyl-Amino Acid Oxidase antibody catalyzes the oxidative deglycation of fructosyl amino acids to produce the corresponding amino acid, glucosone, and hydrogen peroxide. Anti-Fructosyl-Amino Acid Oxidase antibodies are ideal for researchers interested in Metabolism and Signal Transduction research. Synonyms: Fructosyl-amino acid oxidase EMBL BAB91123.1 EC=1.5.3
    UniProt
    Q8RIU8
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