Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)
Cross-Reactivity (Details)
Cross reactivity with HDAC2 from other sources has not been determined.
Purity
Anti-HDAC2 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HDAC2. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, and Primate based on 100 % sequence homology.
Endotoxin Level
Low Endotoxin : No
Immunogen
HDAC2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus region of human HDAC2. Immunogen Type: Peptide
Anti-HDAC2 antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~59 kDa corresponding to the appropriate cell lysate or extract. ELISA Dilution: 1:20.000 - 1:60.000 Immunohistochemistry Dilution: 1:100-1:500 IF Microscopy Dilution: 1:100-1:500 Western Blot Dilution: 1 µg/mL
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 50 % (v/v) Glycerol
Storage
4 °C/-20 °C
Storage Comment
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date
Expiration date is six (6) months from date of opening.
Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDAC2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human. Anti-HDAC2 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Chromatin Research, Epigenetics, Histone Deacetylases, and Stem Cell Markers research. Synonyms: EC 3.5.1.98, HD2, histone deacetylase 2, RPD3, transcriptional regulator homolog RPD3, YAF1, YY1-associated factor 1