BRAF antibody

Catalog No. ABIN1685167

The Rabbit Polyclonal anti-BRAF antibody is suitable to detect BRAF in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for BRAF antibody (ABIN1685167)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-BRAF Antibody

Purification: Purified

Sterility: Sterile filtered

Immunogen: Polyclonal BRAF antibody was produced from sera of rabbits immunized with synthetic protein containing BRAF antigenic determinants.

Isotype: IgG

Application Details

Application Notes: ELISA: Use at a concentration range of 1-2 µg/mL.
Western blot: A suitable range of concentrations of this antibody for WB detection is 10 µg/mL.
IHC: A suitable range of concentrations of this antibody for IHC is 10 µg/mL. Immunohistochemical analysis of BRAF expression in paraffin embedded formalin fixed Rat brain tissue. ABIN1685167 was used as primary antibody, dilution 1:200.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

Buffer: PBS solution at a concentration of 1 mg/mL.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Recombinant human CD117, as supplied, can be stored in working aliquots at - 8 °C for one month, or at -20 °C to -70 °C for twelve months.

Expiry Date: 12 months

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF

Background: The c-Kit receptor consists of an extracellular portion with five immunoglobulin-like regions and an intracellular tyrosine kinase domain. The first three immunoglobulin-like domains form a binding site for its ligand, stem cell factor (SCF), the fourth is involved in c-Kit receptor dimerization, and the function of the fifth is, as yet, unknown. Binding of SCF induces homodimerization of the c-Kit receptor, followed by an increase in receptor autophosphorylation activity and recruitment of the c-Kit signaling complex.

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy