Blood Group Lewis A (AA 201-300) antibody (HRP)
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- Target
- Blood Group Lewis A
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Binding Specificity
- AA 201-300
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- HRP
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Application
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))
- Predicted Reactivity
- Human
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human Blood Group Lewis a
- Isotype
- IgG
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- Application Notes
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WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Blood Group Lewis A
- Synonyms
- CD174 antibody, FT3B antibody, FucT-III antibody, LE antibody, Les antibody, fucosyltransferase 3 (Lewis blood group) antibody, FUT3 antibody
- Background
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Synonyms: Lewis A antigen.
Background: Glycosyltransferases that mediate the regio- and stereoselective transfer of sugars, such as the fucosyltransferases, determine cell surface-carbohydrate profiles, which is an essential interface for biological recognition processes. Fucosyltransferases catalyze the covalent association of fucose to different positional linkages in sugar acceptor molecules. The carbohydrate moieties generated and covalently attached to cell surfaces are necessary to ensure a surface contour that satisfies physiological roles, which are reliant on adhesion molecules such as Selectins (1-3). Hematopoietic lineages rely on Fucosyltransferases to confer a surface carbohydrate phenotype, which mediates proper cell adhesion molecule recruitment and cell trafficking (4-6). Blood Group Lewis a is a carbohydrate determinant carried on both glycolipids and glycoproteins.
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