Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Specificity
Reacts with human ADAM10. Predicted to cross-reacts with bovin, mouse and rat protein.
Purification
Purified
Immunogen
Synthetic peptide corresponding to amino acids 732 to 748 of human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid.
ADAM10
Reactivity: Human
WB, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Working dilution: Optimal dilutions should be determined by the end user. The following are guidelines only: ELISA1:100-1:2000 ICC/IF2 μg/mL IHC-P1:500 WB 1 - 10 μg/mL
Positive control: K562 or Jurkat cells
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.4
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Dilute in PBS or medium which is identical to that used in the assay system.
Storage
-20 °C
Storage Comment
Store in aliquots at -20°C. Antibodies are guaranteed for 6 months from date of receipt.