PTPRF antibody (AA 1315-1607) (Atto 488)

Catalog No. ABIN1740983

Product Details anti-PTPRF Antibody (Atto 488)

Target: PTPRF (Protein Tyrosine Phosphatase Receptor Type F (PTPRF))

Binding Specificity: AA 1315-1607

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PTPRF antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Detects ~85 kDa (full length protein is 210 kDa - smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR. 97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK.

Clone: S165-38

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1740983 was sufficient for detection of LAR/PTPRF in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for PTPRF

Target: PTPRF (Protein Tyrosine Phosphatase Receptor Type F (PTPRF))

Alternative Name: LAR (PTPRF Products)

Synonyms: LAR antibody, AA591035 antibody, LARS antibody, RPTP-LAR antibody, LARFN5C antibody, zf-LAR antibody, protein tyrosine phosphatase, receptor type F antibody, protein tyrosine phosphatase, receptor type, F antibody, protein tyrosine phosphatase, receptor type, f, a antibody, PTPRF antibody, Ptprf antibody, ptprfa antibody

Background: PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule. PTPRF removes phosphate group from β-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function (1,2,3,4). Anti-PTPRF (C-terminal) antibody is specific for the extracellular and cytoplasmic subunits of human PTPRF (approx. 210, 150 and 85 kDa). Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.

Gene ID: 5792

UniProt: P10586

Pathways: EGFR Signaling Pathway