VAMP2 antibody (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2)) (AA 2-17)

Details for Product anti-VAMP2 Antibody No. ABIN1742194, Supplier: Log in to see
Antigen
  • zgc:73302
  • wu:fj61c06
  • VAMP2
  • vamp2-a
  • xsybi
  • Syb-2
  • Syb2
  • sybII
  • RATVAMPB
  • RATVAMPIR
  • SYB
  • SYB2
  • VAMP-2
  • syb2
  • vamp2
  • xsybii
  • vesicle-associated membrane protein 2
  • vesicle associated membrane protein 2
  • vesicle associated membrane protein 2 S homeolog
  • vesicle-associated membrane protein 2 (synaptobrevin 2)
  • vesicle associated membrane protein 2 L homeolog
  • vamp2
  • VAMP2
  • vamp2.S
  • Vamp2
  • vamp2.L
Alternatives
anti-Mouse (Murine) VAMP2 antibody for Immunoprecipitation
Epitope
AA 2-17
30
6
4
3
2
2
2
2
1
1
1
1
1
Reactivity
Human, Rat (Rattus), Mouse (Murine), Hamster
97
43
39
5
3
3
2
2
2
Host
Guinea Pig
54
44
4
4
3
Clonality
Polyclonal
Conjugate
This VAMP2 antibody is un-conjugated
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
94
61
55
26
10
9
8
6
3
3
2
1
Options
Supplier
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Supplier Product No.
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Immunogen Synthetic peptide SATAATVPPAAPAGEG (aa 2-17 in rat synaptobrevin 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for VAMP 2
Cross-Reactivity (Details) no cross reactivity to VAMP 1 and VAMP 3
Purification antiserum
Plasmids, Primers & others Plasmids, Primers & others VAMP2 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Synaptobrevin 2 (VAMP2 Antibody Abstract)
Background Synonyms: VAMP 2, p18-2
Pathways Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
Application Notes WB: 1 : 1000 up to 1 : 5000 (AP staining)
ICC: 1 : 1000
IHC: 1 : 500
Restrictions For Research Use only
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 2-17) antibody (ABIN1742194) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 2-17) antibody (ABIN1742194) Immunocytochemistry on cultured hippocampus neurons (dilution 1 : 1000)
Immunohistochemistry (IHC) image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 2-17) antibody (ABIN1742194) Indirect immunolabeling of PFA fixed mouse hippocampus sections (dilution 1 : 500)
Product cited in: van Stegen, Dagar, Gottmann: "Release activity-dependent control of vesicle endocytosis by the synaptic adhesion molecule N-cadherin." in: Scientific reports, Vol. 7, pp. 40865, 2017 (PubMed).

Schaefer, Toral, Velez, Cox, Baker, Borcherding, Colgan, Bondada, Mashburn, Yu, Geddes, Tsang, Bassuk, Mahajan: "Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses." in: Investigative ophthalmology & visual science, Vol. 57, Issue 6, pp. 2509-21, 2016 (PubMed).

Kung, Gong, Adedoyin, Ng, Bhargava, Ohara, Jasmin: "Evidence for glutamate as a neuroglial transmitter within sensory ganglia." in: PLoS ONE, Vol. 8, Issue 7, pp. e68312, 2013 (PubMed).

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