SYT2 antibody (Lumenal Region)
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- Target See all SYT2 Antibodies
- SYT2 (Synaptotagmin II (SYT2))
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Binding Specificity
- AA 1-11, Lumenal Region
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Reactivity
- Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SYT2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificity
- Specific for synaptotagmin 2.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogen
- Synthetic peptide (aa 1-11 in mouse synaptotagmin 2) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine.
- Top Product
- Discover our top product SYT2 Primary Antibody
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- Application Notes
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WB: 1 : 1000 (AP staining)
ICC: 1 : 100
IHC: not tested yet - Comment
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These antibodies can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Storage
- -20 °C
- Storage Comment
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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- Target
- SYT2 (Synaptotagmin II (SYT2))
- Alternative Name
- Synaptotagmin 2 (SYT2 Products)
- Synonyms
- SytII antibody, RATSYNII antibody, SYNII antibody, R74640 antibody, mKIAA4194 antibody, si:ch211-236j4.1 antibody, synaptotagmin 2 antibody, synaptotagmin II antibody, synaptotagmin IIa antibody, SYT2 antibody, Syt2 antibody, syt2a antibody, syt2 antibody
- Pathways
- Synaptic Vesicle Exocytosis
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