SYT2 antibody (Lumenal Region)

Catalog No. ABIN1742203

Product Details anti-SYT2 Antibody

Target: SYT2 (Synaptotagmin II (SYT2))

Binding Specificity: AA 1-11, Lumenal Region

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Specific for synaptotagmin 2.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide (aa 1-11 in mouse synaptotagmin 2) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 100
IHC: not tested yet

Comment:

These antibodies can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Target Details for SYT2

Target: SYT2 (Synaptotagmin II (SYT2))

Alternative Name: Synaptotagmin 2 (SYT2 Products)

Synonyms: SytII antibody, RATSYNII antibody, SYNII antibody, R74640 antibody, mKIAA4194 antibody, si:ch211-236j4.1 antibody, synaptotagmin 2 antibody, synaptotagmin II antibody, synaptotagmin IIa antibody, SYT2 antibody, Syt2 antibody, syt2a antibody, syt2 antibody

Pathways: Synaptic Vesicle Exocytosis