Profilin antibody (AA 42-47)

Catalog No. ABIN1742560

Product Details anti-Profilin Antibody

Target: Profilin (PFN)

Binding Specificity: AA 42-47

Reactivity: Birch

Host: Mouse

Clonality: Monoclonal

Conjugate: This Profilin antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: Specific for birch profilin.

Purification: purified IgG

Immunogen: Recombinant birch profilin. Epitope PQFKPQ (aa 42-47)

Clone: 4A6

Isotype: IgG2a

Application Details

Application Notes: WB: 1 : 500 up to 1 : 10000
IHC: not tested yet

Comment:

This antibody has been used successfully with eucaryotic and procaryotic expression vectors using the epitope as a tag (Rudiger et al. 1997).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Profilin antibody (ABIN1742560)

Wiedemann, Giehl, Almo, Fedorov, Girvin, Steinberger, Rüdiger, Ortner, Sippl, Dolecek, Kraft, Jockusch, Valenta: "Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody." in: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29915-21, (1997) (PubMed).

Target Details for Profilin

Target: Profilin (PFN)

Alternative Name: Profilin (PFN Products)

Synonyms: LOC406154 antibody, GB13380 antibody, profilin antibody, PFN antibody, PRF antibody