IKK alpha antibody (Internal Region)

Catalog No. ABIN1781824

This Goat Polyclonal antibody specifically detects IKK alpha in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for IKK alpha antibody (Internal Region) (ABIN1781824)

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IKK alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IKK alpha Antibody

Binding Specificity: Internal Region

Purpose: CHUK

Sequence: EHDHSLSCVV TPQD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 84.6 kDa according to NP_001269.3). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Alternative Name: CHUK

Background: CHUK, conserved helix-loop-helix ubiquitous kinase, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16, I-kappa-B kinase 1, I-kappa-B kinase-alpha, IKK-a kinase, IkB kinase alpha subunit, Nuclear factor NFkappaB inhibitor kinase alpha, TCF-16, inhibitor of nucl

Molecular Weight: Expected molecular weight: 80 kDa

Gene ID: 1147

NCBI Accession: NP_001269

Pathways: PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins