Calreticulin antibody (Internal Region)

Catalog No. ABIN1782157

Product Details anti-Calreticulin Antibody

Target: Calreticulin (CALR)

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: calreticulin

Sequence: HPEIDNPEYS PDPS

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human and Rat Lung lysates (calculated MW of 48.1 kDa according to NP_004334.1). These bands correspond to earlier findings in literature with different antibodies (Conway et al, J Biol Chem. 1995 Jul 14,270(28)

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: CALR (CALR Products)

Synonyms: CRT antibody, RO antibody, SSA antibody, cC1qR antibody, crc antibody, crt antibody, Calregulin antibody, CG9429 antibody, Clr antibody, Dmel\\CG9429 antibody, anon-EST:Liang-2.11 antibody, anon-EST:Liang-2.17 antibody, anon-EST:fe3D7 antibody, clone 2.11 antibody, clone 2.17 antibody, eth antibody, potp antibody, cc1qr antibody, ssa antibody, NV19007 antibody, CRP55 antibody, ERp60 antibody, HACBP antibody, CHOT1 antibody, CHT1 antibody, CT1 antibody, AA589632 antibody, CRTR antibody, Creat antibody, CCDS1 antibody, calrl2 antibody, fc66d10 antibody, wu:fc66d10 antibody, wu:fj50c03 antibody, zgc:66153 antibody, calreticulin antibody, calreticulin L homeolog antibody, Calreticulin antibody, putative calreticulin antibody, solute carrier family 6 member 8 antibody, solute carrier family 6 (neurotransmitter transporter, creatine), member 8 antibody, CALR antibody, calr.L antibody, Crt antibody, crt antibody, CRT antibody, Calr antibody, calr antibody, LMJF_31_2600 antibody, Crc antibody, Slc6a8 antibody, SLC6A8 antibody, LOC8269812 antibody

Background: CALR, calreticulin, CRT, RO, SSA, cC1qR, CRP55, ERp60, HACBP, Sicca syndrome antigen A (autoantigen Ro, calreticulin), calregulin, endoplasmic reticulum resident protein 60, grp60

Molecular Weight: Expected molecular weight 60 kDa

Gene ID: 811, 12317, 64202

NCBI Accession: NP_004334

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response