Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Specificity
Highly specific for HSF1. Detects an ~85 kDa protein in unstressed cell lysates, and an ~95 kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.
Cross-Reactivity (Details)
Species reactivity (expected):Human, Rat, Bovine, Guinea Pig, Hamster, Monkey, RabbitSpecies reactivity (tested):Mouse
Immunofluorescence. Immunoprecipitation (Native only): use Protein G and Ab at 2g/mgprotein lysate). Western Blotting: Ab 1-2g/mL for 2hrs at RT. Recommended positivecontrol: LS174T or MAD109 Cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.2 mg/mL
Buffer
PBS pH 7.4, 50 % Glycerol, 0.09 % Sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at 2-8 °C for up to one month or in aliquots at -20 °C for longer. Avoid repeated freezethaw cycles! Shelf life: one year from despatch.
HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents, binds to heat shock response elements (HSEs) in the upstream region of heat shock gene and activates their transcription. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA.Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1